Safranin O stain for cartilage requires a specific procedure involving fixative, deparaffinization, staining, and mounting. The use of acetic acid after staining sets the stain to the tissue.
S8884
Safranin O
Dye content, ≥80%, certified by the Biological Stain Commission, powder
Sinónimos:
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
25 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 114,00 € |
100 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 387,00 € |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C20H19ClN4
Número CAS:
Peso molecular:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
207-518-8
MDL number:
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
114,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleNombre del producto
Safranin O, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder
composition
Dye content, ≥80%
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
General description
Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.
Application
Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.
Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) titration: suitable | technique(s) microbe id | staining: suitable |
| color green to very dark green | color - | color - | color red to very dark red |
| form powder | form powder | form powder or crystals | form liquid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility H2O: 1 mg/mL | solubility water: 1 mg/mL, clear, dark red to very dark red and red purple | solubility methanol: 0.01 g/10 mL, red | solubility water: soluble at 20 °C |
| composition Dye content, ≥80% | composition Dye content, ≥85% | composition - | composition ethanol, 10% |
Still not finding the right product?
Explore all of our products under Safranin O
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
Is there a staining protocol available for staining cartilage, particularly for decalcified bone (joint tissue) using CalX, and subsequent staining with Fast green and Safranin O?
1 respuesta-
¿Le ha resultado útil?
-