This product is provided as a 5000X concentration in DMSO. The specific concentration of this dye is considered proprietary.
S5692
SYPRO™ Orange Protein Gel Stain
Sinónimos:
protein gel stain, SYPRO™ dye
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
50 μL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 98,70 € |
500 μL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 496,00 € |
Acerca de este artículo
UNSPSC Code:
41105322
EC Number:
200-664-3
NACRES:
NA.32
98,70 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleshelf life
≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)
Quality Segment
technique(s)
protein staining: suitable
fluorescence
λex 300,470 nm; λem 570 nm
General description
SYPRO Orange is similar to SYPRO Red although it is somewhat brighter and gives slightly higher background fluorescence. For those using a laser-excited gel scanner, an argon-ion laser-based instrument is recommended for SYPRO Orange. The dye is efficiently excited by UV or broad band illumination. It is not suitable for staining proteins on blots or in IEF gels and shows reduced sensitivity when staining proteins on 2-D gels.
SYPRO Orange is:
SYPRO Orange is:
- Highly sensitive. The stain can detect 1 to 2 ng of protein per minigel band, making it more sensitive than Coomassie Brilliant Blue or silver staining.
- Rapid. Staining is complete in less than one hour
- Simple. After electrophoresis, the gel is stained, rinsed and photographed; no fixation or destaining steps are required
- Compatible with standard laboratory equipment. Stained proteins can be visualized with a standard 300 nm UV transilluminator or laser scanner.
- Cost-effective. Staining with SYPRO Orange is less expensive than silver staining and requires less time.
- Low protein-to-protein variability. The dye interacts with the SDS coat around the protein, giving more consistent staining between different types of proteins compared to Coomassie or silver staining.
- Selective for proteins. SYPRO Orange detects proteins as small as 6.5 kDa and does not stain nucleic acids or lipopolysaccharides. It does stain glycosylated proteins.
- Broad linear range of detection. The fluorescence intensity of the stained bands is linear with protein quantity over three orders of magnitude (a much broader range than either Coomassie or silver staining).
Application
SYPRO orange protein gel stain has been used in the thermal denaturation assay and thermal shift assay of protein(s) of interest.[1] It has also been used in differential scanning fluorimetry.
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
The SYPRO stock solutions should be stored desiccated and protected from light at room temperature, 4 °C or 20 °C. When stored properly, these stock solutions are stable for six months to one year. The staining reagent diluted in buffer or acetic acid solution can be stored in very clean and detergent-free glass or plastic bottles, protected from light at 4 °C for at least three months.
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Este artículo | |||
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) PCR: suitable | technique(s) - |
| fluorescence λex 300,470 nm; λem 570 nm | fluorescence λex 280,450 nm; λem 610 nm | fluorescence - | fluorescence - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life - | shelf life - |
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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サイプロオレンジ s5692 50ul の濃度は5mMですか？
1 respuesta-
¿Le ha resultado útil?
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It's used for Protein quantification assay?
1 respuesta-
This reagent is suitable for the detection of proteins. However, protein quantitation typically utilizes other methods, as described in the graphic below. Please see the following link to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/161/599/s5692dat.pdf¿Le ha resultado útil?
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1. Is the SYPRO Orange Protein Gel Stain a 5,000x concentrate?
2. Is there a protocol available for using the SYPRO Orange protein stain for a thermal denaturation assay?1 respuesta-
The SYPRO Orange Protein Gel Stain is indeed provided as a 5000X concentrated solution in DMSO.
Regarding a protocol for using the SYPRO Orange protein stain for a thermal denaturation assay, the product information is focused on gels. While the product is not specifically tested for this application,¿Le ha resultado útil?
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