RECOMT
Thrombin CleanCleave™ Kit
Sinónimos:
Thrombin Cleavage Kit
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.56
Productos recomendados
formulario
suspension
Nivel de calidad
mol peso
37 kDa
técnicas
protein purification: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Aplicación
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Características y beneficios
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Cantidad
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Información legal
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Solo componentes del kit
Referencia del producto
Descripción
- Thrombin Cleavage Buffer, 10× 10 mL
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Protocolos
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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