RECOMT
Thrombin CleanCleave™ Kit
Sinónimos:
Thrombin Cleavage Kit
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 562,00 € |
Acerca de este artículo
NACRES:
NA.56
UNSPSC Code:
12352200
562,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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suspension
Quality Segment
mol wt
37 kDa
technique(s)
protein purification: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Application
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Features and Benefits
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Preparation Note
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Legal Information
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Este artículo | |||
|---|---|---|---|
| technique(s) protein purification: suitable | technique(s) protein extraction: suitable | technique(s) - | technique(s) - |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| mol wt 37 kDa | mol wt - | mol wt - | mol wt 37.4 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| form suspension | form - | form - | form aqueous solution |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level 200 |
Solo componentes del kit
Referencia del producto
Descripción
- Thrombin Cleavage Buffer, 10× 10 mL
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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