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RAB0657

Human FABP3 / Fatty Acid-binding Protein, Heart ELISA Kit

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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... FABP3(2170)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.5 ng/mL
standard curve range: 2.05-200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FABP3(2170)

General description

Fatty acid-binding protein-3 (FABP3) is a small cytoplasmic protein, which is a member of the multigene family of FA-binding proteins. It is widely expressed in the heart. This gene is mapped to human chromosome 1p35.
SRC family belongs to non-receptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. SRC is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Fatty acid-binding protein-3 (FABP3) participates in cardiac metabolism. It plays an important role in the intracellular uptake of fatty acids and their migration towards the mitochondrial β-oxidation system.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0537RAB1445RAB1446
Gene Information

human ... FABP3(2170)

Gene Information

human ... FABP2(2169)

Gene Information

human ... FABP5(2171)

Gene Information

human ... FABP6(2172)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.5 ng/mL
standard curve range: 2.05-200 ng/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 25 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12.5 ng/mL
standard curve range: 12.50-800 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.64 ng/mL
standard curve range: 1.638-400 ng/mL

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type plasma
sample type serum
sample type urine
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric


Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Lot/Batch Number

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SKUGTIN
RAB0657-1KT04061835164486

Preguntas

  1. I would like to request ELISA instructions for Fatty Acid-binding Protein, Heart ELISA Kit (RAB0657). Our team has this ELISA kit but there was no instruction or information about the concentration of the standard needed for the calibration curve

    1 respuesta
    1. The protocol is included in the Certificate of Analysis. Please view the sample Certificate of Analysis below:
      https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0657_0242F0060.pdf

      A general ELISA protocol (not specific to this kit) can also be found via this link below:
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/elisa/elisa-protocols

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