The protocol is included in the Certificate of Analysis. Please view the sample Certificate of Analysis below:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0657_0242F0060.pdf
A general ELISA protocol (not specific to this kit) can also be found via this link below:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/elisa/elisa-protocols




