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RAB0366

Mouse MMP-2 ELISA Kit

for serum, plasma and cell culture supernatant

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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Mmp2(17390)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Mmp2(17390)

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RAB0365RAB0373RAB0368
species reactivity

mouse

species reactivity

human

species reactivity

mouse

species reactivity

mouse

Gene Information

mouse ... Mmp2(17390)

Gene Information

human ... MMP2(4313)

Gene Information

mouse ... Mmp9(17395)

Gene Information

mouse ... Mmp3(17392)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 27.43-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3,500 pg/mL
standard curve range: 3.3-800 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 13.72-10000 pg/mL

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

Matrix Metallopeptidase 2 (MMP-2), also known as gelatinase A, belongs to the gelatinase subclass of the matrix metalloproteinase (MMP) family. It contains zinc in the active site.[1] MMP-2 cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, vitronectin, elastin, laminin, proteoglycans, and a range of non-extracellular matrix (ECM) components.[2][1] Both MMP-2 and MMP9 are important for matrix degradation and they are implicated in the maintenance of neovascularization.[3] The Mouse MMP-2 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP-2 in serum, plasma, cell culture supernatants, and urine.

Immunogen

Recombinant Mouse MMP2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse MMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify matrix metalloproteinase -2 (MMP-2) secreted in supernatants by gold nanoparticles (AuNP)-loaded endothelial cells[4] and superparamagnetic iron oxide nanoparticle (SPION) loaded endothelial cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Visite la Librería de documentos

Takayuki Shiomi et al.
Pathology international, 60(7), 477-496 (2010-07-03)
Cellular functions within tissues are strictly regulated by the tissue microenvironment which comprises extracellular matrix and extracellular matrix-deposited factors such as growth factors, cytokines and chemokines. These molecules are metabolized by matrix metalloproteinases (MMP), a disintegrin and metalloproteinases (ADAM) and
J C Rodriguez-Manzaneque et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12485-12490 (2001-10-19)
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression, we generated mammary tumor-prone mice that either lack, or specifically overexpress, TSP1 in the mammary
Subhadeep Chakrabarti et al.
Experimental lung research, 31(6), 599-621 (2005-07-16)
The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of
Disturbance of adhesomes by gold nanoparticles reveals a size- and cell type-bias.
Mulens-Arias, et al.
Biomaterials Science, 7, 389-408 (2019)
Theranostic iron oxide nanoparticle cargo defines extracellular vesicle-dependent modulation of macrophage activation and migratory behavior
Mulens-Arias, V, et al.
Advanced Biosystems, 1800079-1800079 (2018)

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SKUGTIN
RAB0366-1KT04061834961970

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