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RAB0210

Sigma-Aldrich

Mouse Granzyme B ELISA Kit

for serum, plasma and cell culture supernatant

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1 KIT
624,00 €

624,00 €


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1 KIT
624,00 €

About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

624,00 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

reactividad de especies

mouse

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
capture ELISA: suitable

entrada

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

mouse ... Gzmb(14939)

Categorías relacionadas

Descripción general

The Mouse Granzyme B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Granzyme B in serum, plasma, cell culture supernatants and urine.
The Mouse Granzyme B enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of mouse granzyme B in serum, plasma, cell culture supernatants and urine. Granzyme B binds to mannose-6-phosphate/insulin-like growth factor II receptor. This protein is implicated in fast DNA fragmentation, a key step towards apoptosis in cells. Granzyme B is involved in the cleaving of pro-caspase-3, which leads to the activation of caspase-3. This protein also cleaves caspase-6, -7, -8, -9, and -10.[1]

Inmunógeno

Recombinant Mouse Granzyme B

Aplicación

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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Immunological reviews, 193, 31-38 (2003-05-20)
A main pathway used by cytotoxic T lymphocytes (CTLs) and natural killer cells to eliminate pathogenic cells is via exocytosis of granule components in the direction of the target cell, delivering a lethal hit of cytolytic molecules. Amongst these, granzyme

Preguntas

  1. It says here that this ELISA kit works for the detection of GzmB in mouse serum, but I can't find any papers that have used serum. Could you please cite a paper or previous work demonstrating the utility of this kit for detecting GzmB in serum?

    1 respuesta
    1. Unfortunately, published citations on the use of this kit with mouse serum are not available. Please see the image below for an example of expected recovery rates:

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