The Human FGF-7 ELISA kit has not been tested for cross-reactivity to mouse, so we cannot confirm if it can detect mouse FGF-7. However, we do offer a Mouse FGF-7 ELISA with the catalog number RAB1123.
RAB0188
Human FGF-7 ELISA Kit
for serum, plasma, cell culture supernatant and urine
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 539,00 € |
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... FGF7(2252)
Input:
sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum
sample type plasma
sample type urine
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
539,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlespecies reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.55-400 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FGF7(2252)
General description
The gene FGF7 (fibroblast growth factor 7) is mapped to human chromosome 15q21.1. The encoded protein belongs to the fibroblast growth factor family and is secreted by mesenchymal cells.
The Human FGF-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-7 in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Human FGF7
Application
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
FGF7 (fibroblast growth factor 7) is involved in the development of skin and sweat glands, wound healing and cutaneous homeostasis. It controls keratinocyte differentiation and viability by modulating autophagy. FGF7 also participates in embryonic development, tumorigenesis, cell proliferation, migration and differentiation. It is linked with several disorders like nonsyndromic cleft lip and palate, thyroid volume, goiter risk and chronic obstructive pulmonary disease. FGF7 plays an important role in the morphology of developing lungs. Absence of the FGF7 activity decreases airway branching during development. During injury, it is involved in airway repair.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Este artículo | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| Gene Information human ... FGF7(2252) | Gene Information human ... FGF6(2251) | Gene Information human ... FGF9(2254) | Gene Information human ... FGF2(2247) |
| input sample type cell culture supernatant(s) | input sample type cell culture supernatant(s) | input sample type urine | input sample type cell culture supernatant(s) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Clase de almacenamiento
10 - Combustible liquids
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Will the Human FGF-7 ELISA kit (BAB0188) detect mouse FGF7?
1 respuesta-
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What is the specificity of the Human FGF7 ELISA antibodies (RAB0188)?
1 respuesta-
The capture antibody utilized in this kit is a mouse monoclonal antibody with an immunogen of aa32-194 of human FGF-7 (accession # P21781). Meanwhile, the detection antibody is a goat polyclonal antibody with the immunogen of aa32-194 of human FGF-7 (accession # Q6FGV5).
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Is there a detailed protocol for this (the general sandwich protocol is not specific enough)? E.g. the kit FGF-7 concentrate for the standard curve - no concentration is written on the vial. And there is no information on how to make serial dilutions.
1 respuesta-
Using the lot number on the product you purchased, you can access the lot-specific CofA to view the procedure and concentration. Example CofA using lot number 0922J0245: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0188_0922J0245.pdf
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