R0886
Monoclonal Anti-Rat IgM antibody produced in mouse
clone RTM-32, ascites fluid
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About This Item
Productos recomendados
origen biológico
mouse
conjugado
unconjugated
forma del anticuerpo
ascites fluid
tipo de anticuerpo
secondary antibodies
clon
RTM-32, monoclonal
contiene
15 mM sodium azide
técnicas
capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:1,000
western blot: suitable
isotipo
IgG1
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Monoclonal anti-Rat IgM (mouse IgG1 isotype) is derived from the RTM-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat IgM myeloma preparation. Immunoglobulin M (IgM) is the first immunoglobulin to be produced, which is detected as early as 9 weeks of gestation. It has a pentameric structure, in which monomers are linked together by disulfide bonds. IgM is produced by B cells and is expressed in high concentrations in blood.
Rat immunoglobulins have seven distinct classes, namely, IgM, IgA, IgE, IgG1, IgG2a, IgG2b and IgG2c. Rat IgMs have been implicated in complement activation . Rat IgMs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgM is a useful tool for the analysis of target proteins
Monoclonal Anti-Rat IgM antibody is specific for an epitope present on the heavy chain of rat IgM. By immunoblotting, the antibody detects the denatured-reduced heavy chain molecule of rat IgM (μ-chain). The product recognizes rat IgMs derived from normal serum or myeloma proteins, but does not detect other rat immunoglobulins. In assays such as indirect ELISA and dot blot, the antibody weakly associates with guinea pig immunoglobulins. The product does not bind to IgG or serum preparations obtained from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.
Monoclonal Anti-Rat IgM antibody is specific for an epitope present on the heavy chain of rat IgM. By immunoblotting, the antibody detects the denatured-reduced heavy chain molecule of rat IgM (μ-chain). The product recognizes rat IgMs derived from normal serum or myeloma proteins, but does not detect other rat immunoglobulins. In assays such as indirect ELISA and dot blot, the antibody weakly associates with guinea pig immunoglobulins. The product does not bind to IgG or serum preparations obtained from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.
Especificidad
Monoclonal anti-Rat IgM recognizes an epitope located on the heavy chain of rat IgM. The antibody detects the rat IgM derived from normal serum or myeloma proteins, but not the other rat immunoglobulins.
Inmunógeno
Rat IgM myeloma preparation
Aplicación
Monoclonal Anti-Rat IgM antibody is suitable for use in:
- indirect ELISA (1:1,000)
- immunocytochemistry
- immunohistochemistry
- dot blot
- immunoblotting
Acciones bioquímicas o fisiológicas
Immunoglobulin (IgM) serves as the first line of defense during microbial infections. It serves as the antigen receptor of naive B cells. Secreted IgM (sIgM) is implicated in B cell maturation and complement activation. Detection of IgM antibodies in a neonate′s serum may indicate intrauterine infection (e.g. congenital rubella syndrome). Development of anti-donor IgM after combined liver-kidney transplantation provides a graft-protecting effect. Anti-Human IgM (μ-specific) antibodies are used in the detection of IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMID) and the hyper immunoglobulin M (hyper-IgM or HIGM) syndromes.
Forma física
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Almacenamiento y estabilidad
For continuous use, store at 2-8 °C. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 30(3), 233-238 (2004-05-12)
Rubella virus generally causes a mild fever, rash illness similar in clinical presentation to infections by other viruses including measles and parvovirus B19. Rubella infections in pregnant women in the first trimester carry a high risk of congenital rubella syndrome
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The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh)
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Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 97(6), 717-730 (2007-01-05)
To review and compare previously reported cases of selective IgM immunodeficiency (SIgMID) with the largest adult cohort obtained from a retrospective analysis of an allergy and immunology practice. Publications were selected from the English-only PubMed database (1966-2005) using the following
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