R0508

(−)-Riboflavina

acrylamide photopolymerization tested

• Sinónimos: Lactoflavina, Vitamina B₂, Vitamina G
• Fórmula empírica (notación de Hill): C₁₇H₂₀N₄O₆
• Número CAS: 83-88-5
• Peso molecular: 376.36
• EC Number: 201-507-1
• UNSPSC Code: 12352200
• PubChem Substance ID: 57654584
• Beilstein/REAXYS Number: 97825
• MDL number: MFCD00005022

Propiedades

• biological source: synthetic (organic)
• form: powder
• mp: 290 °C (dec.) (lit.)
• suitability: acrylamide photopolymerization tested
• foreign activity: Protease, none detected
• SMILES string: CC1=C(C)C=C(N(C[C@H](O)[C@@H]([C@H](O)CO)O)C(C2=N3)=NC(NC2=O)=O)C3=C1
• InChI: 1S/C17H20N4O6/c1-7-3-9-10(4-8(7)2)21(5-11(23)14(25)12(24)6-22)15-13(18-9)16(26)20-17(27)19-15/h3-4,11-12,14,22-25H,5-6H2,1-2H3,(H,20,26,27)/t11-,12+,14-/m0/s1
• InChI key: AUNGANRZJHBGPY-SCRDCRAPSA-N

Descripción

Application

Riboflavin is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. Riboflavin is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)