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PCRISPR001

Sigma-Aldrich

Human CRISPR Activation Calabrese Library

Sinónimos:

CRISPR Activation Library, CRISPRa Library

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About This Item

Código UNSPSC:
41105904
NACRES:
NE.02

envase

pkg of 10 vials (5x200µL aliquots of each component)

concentración

≥5x108 VP/ml (via p24 Assay)

aplicaciones

CRISPR

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Aplicación

  • Functional Genomics/Target Validation
  • Focused forward genetic activation screening
  • Validated positive and negative controls

Características y beneficios

  • Focus on your research, and we will generate your lentivirus screening library
  • Compatible with SAMHELPERV for consistent effector expression across a wide variety of cell lines dCas9-VP64-Blasticidin MS2-P65-HSF1-Hygromycin
  • Targeting Genes 18,885 (Set A), 18,843 (Set B)
  • Compact Libary with gRNAs56,762 (Set A), 56,476 (Set B)
  • Built-in Controls 500, unique non-targeting controls are in each of the two half-libraries (Set A and Set B)

Componentes

2 Subpools with a minimum concentration of 5x108 VP/mL(via p24 assay)
PCRISPR001A - Human CRISPR Activation Calabrese Library Set A
PCRISPR001B - Human CRISPR Activation Calabrese Library Set B

Principio

The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation.

CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number.

Información legal

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Kendall R Sanson et al.
Nature communications, 9(1), 5416-5416 (2018-12-24)
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene function. Here, we show that our recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing

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