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P7943

L-α-Phosphatidylethanolamine from egg yolk

Type III, ≥97% (TLC), lyophilized powder

Sinónimos:

1,2-Diacyl-sn-glycero-3-phosphoethanolamine, L-α-Cephalin

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A ustedes/SKUDisponibilidadPrecio
5 mg
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102,00 €
25 mg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
336,00 €
252,00 €
100 mg
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904,00 €
678,00 €

Acerca de este artículo

Número CAS:
UNSPSC Code:
51191904
NACRES:
NA.25
EC Number:
254-438-4
MDL number:

102,00 €


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biological source

egg yolk

Quality Segment

type

Type III

assay

≥97% (TLC)

form

lyophilized powder

functional group

phospholipid

lipid type

phosphoglycerides

shipped in

ambient

storage temp.

−20°C

SMILES string

[P](=O)(OC[C@H](OC(=O)CCCCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCC)(OCCN)O

InChI

1S/C40H80NO8P/c1-3-5-7-9-11-13-15-17-18-19-20-21-23-25-27-29-31-33-40(43)49-38(37-48-50(44,45)47-35-34-41)36-46-39(42)32-30-28-26-24-22-16-14-12-10-8-6-4-2/h38H,3-37,41H2,1-2H3,(H,44,45)/t38-/m1/s1

InChI key

NJGIRBISCGPRPF-KXQOOQHDSA-N

General description

L-α-Phosphatidylethanolamine from egg yolk has high unsaturated fatty acid content and elicits radical-scavenging activity. L-α-Phosphatidylethanolamine (PE) is a membrane phospholipid and is interconverted to phosphotidylserine in the presence of phosphatidylserine synthase 2. PE is essential for maintaining the structural integrity of membranes. It is the second most abundant phospholipid in animals, plants and yeast and the major lipid in bacteria. It is synthesized in mitochondria in mammals.

Application

L-α-Phosphatidylethanolamine from egg yolk has been used:
  • as a component of lipid mixture for the synthesis of giant unilamellar vesicles (GUVs)
  • in ceramide phosphoethanolamine (CPE) synthase assay using crude sphingomyelin synthase-related protein from Hela cells
  • for the standard curve generation for the quantification of lipids in plantaris muscle homogenates from mice

Packaging

Packaged under Argon.

Disclaimer

Purity when prepared is 97-99%. Solutions exposed to room temperature can decompose 0.3-0.5% per day.

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Este artículo
P3556P6386P8318
functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

biological source

egg yolk

biological source

egg yolk

biological source

egg yolk

biological source

egg yolk

assay

≥97% (TLC)

assay

≥99% (TLC)

assay

≥97%

assay

≥99% (TLC)

shipped in

ambient

shipped in

ambient

shipped in

dry ice

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

lyophilized powder

form

lyophilized powder

form

solution

form

lyophilized powder


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certificados de análisis (COA)

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Preguntas

1–3 de 3 Preguntas  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      ¿Le ha resultado útil?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      ¿Le ha resultado útil?

  3. What solvents are used to dissolve phosphatidylethanolamine?  What is the solubility?  What solvent is.least toxic to eucaryotic cells?

    1 respuesta
    1. This solubility is tested in CHCl3 at 25 mg/mL. Stock solutions in chloroform can be added to medium or buffer and allowed to stir prior to filtration. This allows the chloroform to evaporate off while keeping the Phosphatidylethanolamine in solution, thereby avoiding the risk of exposing cells to the solvent. An alternative solvent would be a mixture of chloroform and methanol, which would also be cytotoxic. Please see the sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7943-BULKSLCN2430.pdf

      ¿Le ha resultado útil?

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