P6101

Anti-Platelet-Derived Growth Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Sinónimos: Anti-PDGF
• Número MDL: MFCD00165456
• Código UNSPSC: 51111800
• NACRES: NA.41

Propiedades

• origen biológico: goat
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: lyophilized powder
• reactividad de especies: human
• técnicas: indirect ELISA: suitable; neutralization: suitable; western blot: suitable
• Nº de acceso UniProt: P01127; P04085
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PDGFA(5154) , PDGFB(5155)

Descripción

Descripción general

Platelet-derived growth factor (PDGF) is a serum growth factor purified from human platelets. The members of PDGF are homodimers of A-, B-, C- and –D polypeptide chains and an AB heterodimer. PDGF is a mitogen and stimulates the proliferation of cells of connective tissue and glial cells, survival, differentiation, chemotaxis and wound healing. The cellular effects of PDGF isoforms are mediated by binding to α- and β-receptors which leads to activation of downstream signaling via PI3K, STAT and Ras/MAPK. Overexpression of PDGF isoforms has been observed in various malignancies including gliomas, osetosarcoma, chronic myelomonocytic leukemia and non-small-cell lung cancers
Anti-Human Platelet-Derived Growth Factor (PDGF) specifically recognizes natural human PDGF. It will also neutralize the activity of recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. It shows cross reactivity with recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB.

Especificidad

The antibody will neutralize the biological activity of natural human PDGF, recombinant human PDGF-AB, PDGF-AA and PDGF-BB and natural porcine PDGF-BB.

Inmunógeno

natural human PDGF.

Aplicación

Anti-Human Platelet-Derived Growth Factor (PDGF) antibody may be used for ELISA at a working concentration of 0.25 μg/ml. The antibody has been used for ELISA at a dilution of 1:500 using human serum.. For immunoblotting, a concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions and has been used to neutralize PDGF of primary Human dermal fibroblasts (HDFs) and human platelet-enriched plasma.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) with 5% trehalose.

Nota de preparación

Purified using protein G.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)