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P4600

Sigma-Aldrich

ReadyMix™ Taq PCR Reaction Mix

with MgCl2

Sinónimos:

PCR master mix, Taq PCR master mix

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About This Item

Número MDL:
MFCD01635810
Código UNSPSC:
41106300
NACRES:
NA.55

formulario

liquid

uso

sufficient for 100 reactions

Características

dNTPs included
hotstart: no

concentración

1.5 units/reaction (50 μL reaction volume)

técnicas

PCR: suitable

color

colorless

entrada

purified DNA

aplicaciones

agriculture

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Descripción general

ReadyMix™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Aplicación

ReadyMix™ Taq PCR Reaction Mix has been used:
  • in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers
  • in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis
  • in the molecular identification of yeasts
  • for routine PCR amplifications

Características y beneficios

  • Amplifies targets up to 7 kb in length

  • ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

  • ReadyMix format reduces setup time.

  • Reduced risk of contamination from multiple pipet steps.

Envase

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
Supplied with a vial of PCR grade water for dilution.

Definición de unidad

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Información legal

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Producto relacionado

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Assessment of fecal microflora changes in pigs supplemented with herbal residue and prebiotic
Samanta A K, et al.
PLoS ONE, 10(7), e0132961-e0132961 (2015)
"RNA walk" a novel approach to study RNA?RNA interactions between a small RNA and its target.
Lustig Y, Wachtel C, Safro M, Liu L and Michaeli S
Nucleic Acids Research, 38, 5-5 (2010)
A Dimitroglou et al.
Journal of animal science, 87(10), 3226-3234 (2009-07-21)
A study was conducted to investigate the effect of mannan oligosaccharide (MOS) on the gut microbiota and intestinal morphology of rainbow trout under commercial farming conditions. Juvenile (mean initial BW 38.2 +/- 1.7 g) and subadult (111.7 +/- 11.6 g)
Ashis Kumar Samanta et al.
PloS one, 10(7), e0132961-e0132961 (2015-07-16)
Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment. The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder)
Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA
Shaked H, Wachtel C, Tulinski P, Yahia NH et al.
Nucleic Acids Research, 38, 114-114 (2010)
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Artículos

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Protocolos

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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