capture ELISA: 5 μg/mL using Platelet extract (Use Sigma Monoclonal Anti-Phosphotyrosine Antibody (Prod. No. P3300)), western blot: 5 μg/mL using Platelet extract (Use Sigma Monoclonal Anti-Phosphotyrosine P-3300)
shipped in
dry ice
storage temp.
−20°C
General description
The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphotyrosine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphotyrosine antibodies but not that of anti-phosphothreonine- or anti-phosphoserine-specific antibodies.
Application
Phosphotyrosine-BSA may be used for specific inhibition of phosphotyrosine in platelet extracts, in immunoblotting and ELISA applications, at a working concentration of 5 μg/mL.
Biochem/physiol Actions
Phosphotyrosine antibody inhibitor that specifically blocks the reactivity of anti-phosphotyrosine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphoserine-specific antibodies.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Analysis Note
O-Phospho-L-tyrosine conjugated to bovine serum albumin
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
