This enzyme is active in the presence of 1% Triton X-100 and also 0.1% SDS.
P2289
Endoproteinase Lys-C from Lysobacter enzymogenes
lyophilized powder
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Número CAS:
UNSPSC Code:
12352204
EC Number:
276-716-4
MDL number:
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lyophilized powder
Quality Level
analyte chemical class(es)
amino acids
packaging
vial of ≥3.0 units
storage temp.
2-8°C
General description
Endoproteinase Lys-C is an enzyme that preferentially cleaves at the carboxyl side of lysine residues. [1]
Application
Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. [1] It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody. [2]
Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment. [3]
Biochem/physiol Actions
Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36.
Other Notes
One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.
signalword
Danger
target_organs
Respiratory system
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
wgk
WGK 1
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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The effects of substrates, products and other ligands on the susceptibility ofinositol monophosphatase to proteolysis by endoprotease lys-C.
Gee NS
FEBS Journal, 284, 95-97 (1991)
Wei Zhang et al.
Analytical biochemistry, 311(1), 1-9 (2002-11-21)
Recombinant monoclonal antibodies (mAbs) are an emerging therapeutic area. However, there are few reports on disulfide bond assignment of recombinant mAbs. This work describes the complete disulfide bond assignment of a recombinant immunoglobulin G4 (IgG4) mAb. N-ethylmaleimide (NEM) was used
Hiromi Ito et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 806(1), 11-17 (2004-05-20)
Formation of covalently bound protein adducts with 2-arylpropionic acids (2-APAs) has been proposed as a possible explanation for hypersensitivity and toxic responses to chiral carboxylic acid drugs. To identify the cellular proteins chemically modified with optically active (S)-ibuprofen, we generate
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Is the protease Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, active in the presence of detergents?
1 respuesta-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289?
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The molecular weight is 30,000 Daltons (30 kDa).
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What amount of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, is present in the 3 unit package size?
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This product is prepared from material with a specific activity of 150 units per mg. Therefore there is approximately 20 micrograms per vial.
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