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OGS1120

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PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

etiqueta

10-His tagged

formulario

buffered aqueous solution

mol peso

size 6201 bp

selección de bacterias

kanamycin

selección de células de mamífero

puromycin

Origen de replicación

pUC (500 copies)

Escisión peptídica

TEV

Ubicación de la etiqueta en el péptido

C-terminal

Promotor

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

gen reportero

none

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

Descripción general

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Secuencia

To view sequence information for this product, please visit the product page

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
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Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
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The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

Artículos

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

Sequencing Primers

Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.

DNA Component Explanation

Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.

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