N2786
Monoclonal Anti-Nedd8 antibody produced in mouse
clone ND23, purified immunoglobulin, buffered aqueous solution
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About This Item
origen biológico
mouse
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
ND23, monoclonal
Formulario
buffered aqueous solution
reactividad de especies
human
técnicas
microarray: suitable
western blot: 0.15-0.3 μg/mL using recombinant GST-Nedd8
isotipo
IgG2b
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... NEDD8(4738)
Descripción general
Monoclonal Anti-Nedd8 (mouse IgG2b isotype) is derived from the ND23 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human Nedd8 conjugated keyhole limpet hemocyanin (KLH). Nedd8 is a small protein (9 kDa) that shows the highest similarity to the ubiquitin protein (60% amino acid sequence identity).
Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is an ubiquitin-like, 76 amino acid protein. It is expressed in endocrine and epithelial cells. NEDD8 belongs to the ubiquitin-like protein superfamily.
Inmunógeno
synthetic peptide corresponding to amino acids 20-32 of human Nedd8, conjugated to KLH.
Aplicación
Monoclonal Anti-Nedd8 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Acciones bioquímicas o fisiológicas
Nedd8 conjugation has a physiological role in processes where the stability of nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and p27 is important.
Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is involved in pathways concerned with protein degradation. It targets substrates for neddylation, which is a post-translational modification. NEDD8 also regulates the signaling of proteins like cullins.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Angela Lucariello et al.
In vivo (Athens, Greece), 27(4), 501-506 (2013-07-03)
Neural precursor cell-expressed, developmentally down-regulated-8 (NEDD8) is a 76-amino-acid ubiquitin-like polypeptide. NEDD8 affects the signaling of various molecules but the major cellular target proteins are cullins. The neddylation process is correlated closely with apoptosis, cell-cycle regulation, embryogenesis and development. The
MLN4924, an NAE inhibitor, suppresses AKT and mTOR signaling via upregulation of REDD1 in human myeloma cells
Gu Y, et al.
Blood, 123(21), 3269-3276 (2014)
Marihan Hegazy et al.
Current protocols in cell biology, 89(1), e115-e115 (2020-10-13)
Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in situ provides important additional information. The Proximity Ligation Assay®
Yanchun Wang et al.
Cancer biology & therapy, 16(3), 420-429 (2015-03-18)
Recent studies indicate that post-translational protein neddylation is required for the maintenance of cell viability in several lymphoma cell lines, while inhibition of the neddylation pathway with an NEDD8-activating enzyme (NAE) inhibitor MLN4924 induces apoptosis in lymphoma cells. However, the
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