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MP0050

Sigma-Aldrich

Lookout® One-Step Mycoplasma PCR Detection Kit

Detection of Mycoplasma Contamination by conventional PCR

Sinónimos:

Mycoplasma Testing Kit

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

recombinante

expressed in E. coli Taq

Nivel de calidad

100

formulario

liquid
lyophilized

Parámetros

2 μL sample volume

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

Lookout® One-Step Mycoplasma PCR Detection Kit is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be dispensed into the reaction tubes according to the sample number. Only samples or controls need to be added prior to PCR.

Especificidad

Type of PCR:
For conventional, endpoint PCR with gel electrophoresis
Result Evaluation: Gel electrophoresis at endpoint of PCR

Aplicación

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Componentes

Kit Components:
Lyophilized Mix (Primer sets / Nucleotides / Internal Control DNA and polymerase at optimized concentrations in aliquots of 25 or 100 reactions each Rehydration Buffer)
Lyophilized Positive Control DNA
PCR Grade Water

Reconstitución

Lyophilized OneStep Mix is reconstituted in 600μl Rehydration Buffer; Lyophilized Positive Control DNA is reconstituted in 300μl PCR Grade Water

Almacenamiento y estabilidad

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.

Otras notas

Required Consumables:
Agarose gels and DNA stains
PCR reaction tubes and pipette filter tips

Required Lab Devices:
PCR cycler for conventional PCR
Gel electrophoresis and documentation system
Pipetting equipment
Microcentrifuge

Información legal

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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