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MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinónimos:

Green PKH membrane mini labeling kit

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

envase

pkg of 1 kit

fabricante / nombre comercial

Distributed for Phanos Technologies

condiciones de almacenamiento

protect from light

técnicas

flow cytometry: suitable

fluorescencia

λex 490 nm; λem 502 nm (PKH67 dye)

aplicaciones

cell analysis
detection

método de detección

fluorometric

Condiciones de envío

ambient

temp. de almacenamiento

room temp

Descripción general

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Aplicación

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Ligadura / enlace

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Información legal

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies

Solo componentes del kit

Referencia del producto
Descripción
  • Diluent C 10 mL
  • PKH67 Cell Linker in ethanol 0.1 mL

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palabra de señalización

Danger

Frases de peligro

H225,H319

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

55.4 °F - closed cup

Punto de inflamabilidad (°C)

13 °C - closed cup

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures
Tseng HY, et al.
Biopreservation and Biobanking, 9(4), 355-362 (2011)
Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization
Huang C, et al.
Molecular Medicine Reports, 18(1), 509-515 (2018)
Haiyang Xu et al.
Stem cell research & therapy, 10(1), 381-381 (2019-12-18)
Mesenchymal stem cells (MSCs) play a significant role in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the effects of exosomal miR-133b derived from MSCs on glioma
Yuan Yang et al.
Experimental and therapeutic medicine, 20(6), 257-257 (2020-11-18)
Mesenchymal stem cell (MSC) infusion has become a novel therapeutic strategy for complex autoimmune diseases; however, few detailed studies have been performed to investigate the benefit and mechanism of MSC treatment on systemic sclerosis (SSc). The present study aimed to
Simone Moertl et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component
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