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Merck

MAK017

Sigma-Aldrich

Lactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

aplicaciones

cosmetics
food and beverages

método de detección

colorimetric
fluorometric

enfermedades relevantes

cancer; neonatal diseases; pediatric diseases

temp. de almacenamiento

−20°C

Descripción general

Lactose is a dissacharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.

Aplicación

Lactose Assay Kit has been used to measure lactose content in milk.

Idoneidad

Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.)

Principio

In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2-1.0 nmole lactose for the fluorometric assay and 2-10 nmoles lactose for the colorimetric assay.

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Referencia del producto
Descripción
Precios

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Effect of high-fat diet on secreted milk transcriptome in midlactation mice.
Chen Y, et al.
Physiological Genomics, 49(12), 747-762 (2017)
Holly DeRosa et al.
eNeuro, 9(4) (2022-08-23)
Breastfeeding confers robust benefits to offspring development in terms of growth, immunity, and neurophysiology. Similarly, improving environmental complexity, i.e., environmental enrichment (EE), contributes developmental advantages to both humans and laboratory animal models. However, the impact of environmental context on maternal
Y Chen et al.
Physiological genomics, 49(12), 747-762 (2017-11-03)
High-fat diet (HFD) during lactation alters milk composition and is associated with development of metabolic diseases in the offspring. We hypothesized that HFD affects milk microRNA (miRNA) and mRNA content, which potentially impact offspring development. Our objective was to determine
Yulie E Meneses et al.
Journal of dairy science, 99(5), 3396-3407 (2016-03-01)
Water scarcity is threatening food security and business growth in the United States. In the dairy sector, most of the water is used in cleaning applications; therefore, any attempt to support water conservation in these processes will have a considerable
Adrienne A Cheng et al.
American journal of physiology. Endocrinology and metabolism, 320(3), E438-E452 (2021-01-12)
Obesity is a prevailing problem across the globe. Women who are obese have difficulty initiating and sustaining lactation. However, the impact of genetics and diet on breastfeeding outcomes is understudied. Here we explore the effect of diet and genotype on

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