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Merck

M8403

Sigma-Aldrich

McCoy′s 5A Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

5A basal medium

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About This Item

Número CE:
Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

product name

McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Aplicación

McCoy′s 5A Medium has been used to maintain:

  • G-361 melanoma cells;
  • 87-MG cells
  • human colon carcinoma cell line HCT116

Acciones bioquímicas o fisiológicas

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Reconstitución

Supplement with 0.22 g/L L-glutamine.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Katsuhiro Uzawa et al.
Scientific reports, 4, 5433-5433 (2014-06-26)
No definitive therapy exists to treat human metastatic tumors. We reported previously that down-regulation of Lin-7C is essential for metastasis of human squamous cell carcinomas (hSCCs). In this study, we investigated the chemical restoration of Lin-7C expression and demonstrated its
Seungman Park et al.
Scientific reports, 9(1), 13286-13286 (2019-09-19)
We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted
Claudia De Fusco et al.
Journal of medicinal chemistry, 64(10), 6814-6826 (2021-04-27)
MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite S-adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for
Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can
A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily

Artículos

McCoy's 5A Modified Media Formulation

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