M8065

O⁶-Methylguanine-DNA Methyltransferase human

≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥10,000 units/mg protein

• Sinónimos: AGT, MGMT
• Número MDL: MFCD03792500
• Código UNSPSC: 12352200

Propiedades

• recombinante: expressed in E. coli
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: buffered aqueous glycerol solution
• actividad específica: ≥10,000 units/mg protein
• mol peso: ~21 kDa; apparent mol wt ~25 kDa by SDS-PAGE
• Nº de acceso UniProt: P16455
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... MGMT(4255)

Descripción

Acciones bioquímicas o fisiológicas

O⁶-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O⁶-alkyl-guanine, primarily O⁶-methylguanine, lesions from damaged DNA.

O⁶-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O⁶-alkyl-guanine, primarily O⁶-methylguanine, lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. Its action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. The cysteine sulfhydryl moiety is not regenerated and therefore, MGMT is frequently classified as a suicide enzyme. Since the reaction irreversibly inactivates the enzyme, the repair capacity for O⁶-methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.

Definición de unidad

One unit is the amount of protein that removes 50% of the methyl groups of 0.5 pmol double stranded DNA oligonucleotide substrate containing an O⁶-methylguanine in 10 minutes at 37 °C.

Forma física

Solution in 50% glycerol containing 20 mM Tris, pH 7.5, 1 mM EDTA, 200 mM NaCl, and 1 mM DTT.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)