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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Sinónimos:

MESA Buffer

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grado

for molecular biology

Línea del producto

BioReagent

formulario

powder

técnicas

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubilidad

H2O: soluble, faintly yellow

actividad extraña

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temp. de almacenamiento

room temp

Categorías relacionadas

Descripción general

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Aplicación

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Componentes

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitución

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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MOPS sodium salt anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD018

MOPS sodium salt

MOPS, Free Acid, Molecular Biology Grade CAS 1132-61-2 is a zwitterionic buffer useful in the pH range of 6.5-7.9. Has a pKa of 7.20 at 25°C.

Millipore

475922

MOPS, Free Acid, Molecular Biology Grade

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD003

MOPS

Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Electrophoresis in agarose and acrylamide gels.
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