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M5257

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

Name: Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver
Brand: SIGMA

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Sinónimos:

L-Malate:NADP⁺ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme

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Número CAS:

9028-47-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-834-8

MDL number:

MFCD00131568

Número CE:

1.1.1.40 (BRENDA, IUBMB)

Specific activity:

10-30 units/mg protein (modified Warburg-Christian)

Biological source:

chicken liver

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Propiedades

biological source

chicken liver

form

ammonium sulfate suspension

specific activity

10-30 units/mg protein (modified Warburg-Christian)

storage temp.

2-8°C

Descripción

General description

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate. [1]

Application

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem/physiol Actions

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Physical form

Suspension in 2.9 M (NH₄)₂SO₄ solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na₄EDTA, pH 6.0

Other Notes

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO_2, and NADPH per min at pH 7.4 at 25 °C.

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Este artículo	M1567	M9004	M2634
Sigma-Aldrich M5257 Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver	Sigma-Aldrich M1567 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M9004 Malic Dehydrogenase from bovine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart
specific activity 10-30 units/mg protein (modified Warburg-Christian)	specific activity ≥600 units/mg protein (biuret)	specific activity 2000-4000 units/mg protein (modified Warburg-Christian)	specific activity 600-1000 units/mg protein (biuret)
biological source chicken liver	biological source -	biological source bovine heart	biological source -
form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension	form buffered aqueous glycerol solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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