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Merck

L6893

Lipopolysaccharides (rough strains) from Escherichia coli F583 (Rd mutant)

Sinónimos:

LPS

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Número CAS:
93572-42-0
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
MFCD00164401

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biological source

Escherichia coli (F583 Rd mutant)

Quality Level

200

form

lyophilized powder

impurities

<3% Protein (Lowry)

color

white to yellow cast

solubility

water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

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Lipopolysaccharides (rough strains) from Escherichia coli F583 (Rd mutant)

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Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc&nbsp;mutant)

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Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)

Lipopolysaccharides from Escherichia coli O55:B5 FITC conjugate

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F8666

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O26:B6 purified by trichloroacetic acid extraction

Sigma-Aldrich

L3755

Lipopolysaccharides from Escherichia coli O26:B6

biological source

Escherichia coli (F583 Rd mutant)

biological source

Escherichia coli (J5 Rc mutant)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O26:B6)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

solubility

water: soluble

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<3% Protein (Lowry)

impurities

<3% Protein (Lowry)

impurities

-

impurities

1-10% Protein (Lowry)

General description

This product is phenol:chloroform:petroleum ether extracted from E. coli F 583 (Rd mutant). The source strain is from a private collection.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Referencia del producto
Descripción
Precios

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000239148
0000223697
0000239148
0000223697
0000202382

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M Worku et al.
Journal of dairy science, 92(7), 3185-3193 (2009-06-17)
The objective of this study was to evaluate the effect of smooth (S) and rough (R) forms of lipopolysaccharide (LPS) on gene expression in bovine blood neutrophils. Isolated neutrophils (10(7) cells/mL) were treated with Escherichia coli LPS serotype O111:B4 (S+R)
Chandan Singh et al.
Molecular microbiology, 114(3), 510-520 (2020-05-29)
The outer membrane is a key virulence determinant of gram-negative bacteria. In Yersinia pestis, the deadly agent that causes plague, the protein Ail and lipopolysaccharide (LPS)6 enhance lethality by promoting resistance to human innate immunity and antibiotics, enabling bacteria to
J S Nielsen et al.
Scandinavian journal of immunology, 75(2), 193-202 (2011-08-23)
Immunosuppression induced by lymphocyte apoptosis is considered an important factor in the pathogenesis of sepsis and has been demonstrated in both animal models of lipopolysaccharide (LPS)-induced endotoxemia and septic patients. As rough-form LPS (R-LPS) has recently been shown to elicit
Yawen Ni et al.
PloS one, 12(2), e0170346-e0170346 (2017-02-09)
Occludin is a key tight junction (TJ) protein in cerebral endothelial cells (CECs) playing an important role in modulating blood-brain barrier (BBB) functions. This protein (65kDa) has been shown to engage in many signaling pathways and phosphorylation by both tyrosine
Iga Kucharska et al.
Biochemistry, 55(36), 5061-5072 (2016-08-18)
Pseudomonas aeruginosa is an opportunistic human pathogen causing pneumonias that are particularly severe in cystic fibrosis and immunocompromised patients. The outer membrane (OM) of P. aeruginosa is much less permeable to nutrients and other chemical compounds than that of Escherichia
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Número de artículo de comercio global

SKUGTIN
L6893-5MG04061833275191
L6893-10MG04061833275184

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