KEM0031

Klenow Fragment

Ultra-pure enzyme for nucleic acid modifications

Propiedades

• grado: for molecular biology
• Análisis: >99% (SDS-PAGE)
• formulario: buffered aqueous solution
• actividad específica: 5,000 U/mg
• concentración: 5,000 U/mL
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

Aplicación

Suitable for:

• DNA blunting by fill-in of 5′ overhang
• Second strand cDNA synthesis
• Sequencing
• Site-specific mutagenesis

Características y beneficios

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

Componentes

Supplied with:KEM0042B (10X Blue Buffer)

Definición de unidad

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.

Forma física

Supplied in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.

Otras notas

Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.

Unit size: 2,500 U

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids