K1757

Keratinocyte Growth Factor human

KGF, recombinant, expressed in E. coli, powder, suitable for cell culture

• Sinónimos: hFGF-7, hKGF, FGF-7, KGF
• Número de CAS: 148348-15-6
• Número MDL: MFCD00212245
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Análisis: ≥95% (SDS-PAGE and HPLC)
• formulario: powder
• potencia: 0.1-15 ng/mL ED₅₀/EC₅₀
• calidad: endotoxin tested
• mol peso: 18.9 kDa
• envase: pkg of 10 and 50 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: 1 EU/μg
• Nº de acceso UniProt: P21781
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... FGF7(2252)

Descripción

Descripción general

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.

Aplicación

Keratinocyte Growth Factor human has been used:

• as a component of epithelial growth medium
• as a positive control for antigen to determine the activity of the antibody
• to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Acciones bioquímicas o fisiológicas

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Nota de análisis

Determined by a cell proliferation assay using 4MBr-5 cells.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves