Yes, the difference between product H3667 and H4522, is that product H3667 is heat inactivated. Any availability questions can be directed to customer service. Please navigate to the link below:https://www.sigmaaldrich.com/support/customer-support.
H3667
Suero humano
Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered
Human Serum
Sinónimos:
plasma humano, suero humano, sueros humanos
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
20 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 135,00 € |
100 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 431,00 € |
Acerca de este artículo
Biological source:
human male plasma (Type AB)
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
135,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
human male plasma (Type AB)
Quality Segment
sterility
sterile-filtered
composition
hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
El suero se requiere principalmente como suplemento para los medios de cultivo celular. Contiene macromoléculas, proteínas transportadoras, factores de fijación y de propagación, nutrientes de bajo peso molecular y hormonas y factores de crecimiento.
Application
El suero humano se ha utilizado como suplemento en los medios de cultivo para monocitos.[1] Se ha utilizado como control en la electroinmunotransferencia para estudiar la expresión de flightless I en suero.
Packaging
El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.
Analysis Note
Analizada la presencia de endotoxinas
Other Notes
No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica la presencia de lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.
Disclaimer
SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source human male plasma (Type AB) | biological source human male plasma (Type AB) | biological source human | biological source human |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities (mycoplasma tested, virus tested), ≤10 EU/mL endotoxin | impurities ≤10 EU/mL Endotoxin |
| composition hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤30 mg/dL | composition - | composition Hemoglobin, ≤25 mg/dL |
Still not finding the right product?
Explore all of our products under Suero humano
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
Is the only difference between H3667 and H4522 that H3667 is heat inactivated whilst H4522 is not? And what makes a big difference in the availability between them?
1 respuesta-
¿Le ha resultado útil?
-
-
Is this tested for Anti HLA antibodies? I am thinking of it’s application as a flow cytometric negative control
1 respuesta-
This product has not been tested for the presence of anti-HLA antibodies.
¿Le ha resultado útil?
-
-
What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
¿Le ha resultado útil?
-
-
Why is serum Heat-Inactivated (HI)?
1 respuesta-
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
¿Le ha resultado útil?
-
-
What is the difference between human serum from AB plasma and human serum from AB clotted whole blood?
1 respuesta-
Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present. The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma
¿Le ha resultado útil?
-