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GUSS

Sigma-Aldrich

β-Glucuronidase Reporter Gene Staining Kit

Sinónimos:

β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.32

uso

 kit sufficient for 100 histochemical assays

Nivel de calidad

200

técnicas

microbe id | staining: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Aplicación

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Características y beneficios

  • Histochemical staining of plant tissues expressing the E. coli GUS enzyme
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

Otras notas

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Solo componentes del kit

Referencia del producto
Descripción
  • 2× Fixation Buffer 25 mL
  • 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
  • Reagent A for gus staining kit 50 mL
  • Reagent B for gus staining kit 200 μL
  • Reagent C for gus staining kit 200 μL

Frases de peligro

H412

Consejos de prudencia

P273 - P501

Clasificaciones de peligro

Aquatic Chronic 3

Código de clase de almacenamiento

11 - Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Kuo-Hsiang Chuang et al.
Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
Li-Ling Lin et al.
International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)
Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs
R A Jefferson et al.
The EMBO journal, 6(13), 3901-3907 (1987-12-20)
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
Ken Naito et al.
Nature, 461(7267), 1130-1134 (2009-10-23)
High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such
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