GE28-4082-61

HiTrap^® MabSelect^™ Xtra

Cytiva 28-4082-61, pack of 5 × 5 mL

• Código UNSPSC: 23151816
• NACRES: NA.56

Propiedades

• ligand: recombinant protein A (E. coli)
• envase: pack of 5 × 5 mL
• fabricante / nombre comercial: Cytiva 28-4082-61
• condiciones de almacenamiento: (20% Ehtanol); (20% Ethanol)
• Parámetros: 42 psi
• lecho tamaño: 16 mm × 25 mm
• lecho volumen: 5 mL
• columna D.I.: 16 mm
• Matriz: highly cross-linked agarose
• tam. prom. pieza: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• Intervalo de trabajo: 3-10
• capacidad: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• idoneidad: suitable for bioprocess medium
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ Xtra columns are prepacked with MabSelect^™ Xtra, a BioProcess medium for capture of monoclonal antbodies from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Aplicación

The increased capacity of the medium is well suited for purification of Mabs from high-level expression feedstock.

Características y beneficios

• Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect^™ medium.
• MabSelect^™ Xtra uses the same recombinant Protein A ligand as MabSelect^™
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap^® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids