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GE17-1128-01

MAbtrap® Kit

Cytiva 17-1128-01, pkg of 1 kit

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About This Item

Código UNSPSC:
13111000
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

envase

pkg of 1 kit

fabricante / nombre comercial

Cytiva 17-1128-01

condiciones de almacenamiento

(20% Ethanol)

Parámetros

<4 mL/min flow rate
42 psi

lecho tamaño

7 mm × 25 mm

lecho volumen

1 mL

columna D.I.

7 mm

matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning

2-9

working range

3-9

capacidad

>1 mg binding capacity (lgG/column)

temp. de almacenamiento

2-8°C

Descripción general

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Aplicación

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose High Performance.

Características y beneficios

  • Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
  • Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose High Performance and enough buffer concentrate for 20 preparations with a syringe.
  • MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

This page shows how to prepare samples for purification with affinity chromatography.

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