GE17-0407-01

HiTrap^® Heparin High Performance

Cytiva 17-0407-01, pack of 1 × 5 mL

• Sinónimos: Heparin Affinity Column
• Código UNSPSC: 23151816
• NACRES: NA.56

Propiedades

• ligand: porcine heparin
• envase: pack of 1 × 5 mL
• fabricante / nombre comercial: Cytiva 17-0407-01
• Parámetros: 42 psi
• lecho tamaño: 16 mm × 25 mm
• lecho volumen: 5 mL
• columna D.I.: 16 mm
• tam. prom. pieza: 34 μm
• cleaning: 5-10
• working range: 5-10

Descripción

Descripción general

HiTrap^® Heparin HP columns are prepacked with Heparin Sepharose^™ High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.

Heparin is coupled to the Sepharose^™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap^® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap^® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.

Características y beneficios

• Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
• Simple operation with a syringe, a pump or a chromatography system such as AKTA design
• Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids