G9295
Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse
clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate Dehydrogenase
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About This Item
conjugado:
peroxidase conjugate
application:
WB
clon:
GAPDH-71.1, monoclonal
reactividad de especies:
rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
citations:
234
técnicas:
western blot: 1:35,000-1:70,000 using A431 total cell extract
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
peroxidase conjugate
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
GAPDH-71.1, monoclonal
Formulario
buffered aqueous solution
mol peso
antigen ~37 kDa
reactividad de especies
rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
no debe reaccionar con
prokaryotes
técnicas
western blot: 1:35,000-1:70,000 using A431 total cell extract
isotipo
IgM
Nº de acceso UniProt
aplicaciones
research pathology
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)
Descripción general
Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.
The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.
Especificidad
The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.
Inmunógeno
rabbit GAPDH.
Aplicación
Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Acciones bioquímicas o fisiológicas
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Clasificaciones de peligro
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
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Punto de inflamabilidad (°C)
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Equipo de protección personal
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