G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Sinónimos: G-6-P-DH, Zwischenferment
• Número de CAS: 9001-40-5
• Comisión internacional de enzimas: 1.1.1.49 (BRENDA, IUBMB)
• Número CE: 232-602-6
• Número MDL: MFCD00081656
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Type IX
• Nivel de calidad: 200
• formulario: lyophilized powder
• actividad específica: 200-400 units/mg protein (modified Warburg-Christian)
• mol peso: 128 kDa
• contenido de β-NADP y de β-NADPH: ≤10 mmol/mol
• aplicaciones: agriculture
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

Aplicación

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.
• For recycling microassay of β-NADP and β-NADPH.
• To measure the intracellular levels of NADPH and total NADP.
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.

Acciones bioquímicas o fisiológicas

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

Definición de unidad

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Forma física

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)