EC996
GenElute™-E Conditioning Plate 96
For use with EC848 and EC896 kits. 2 plates.
Seleccione un Tamaño
Acerca de este artículo
General description
- Isolation of viral RNA and DNA in less than 30 minutes with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, and subject the nucleic acids to centrifugation sheering forces.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in swab, cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminantswhile allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-qualitypurification is made possible by our innovative SmartLyse® Buffer, which enables fast and efficient isolation.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply prepare spin column, mix sample with lysis components, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified viral RNA and DNA is ready to be used in downstream applications or stored.
Application
The sample lysis utilizes the SmartLyse® Viral Buffer for fast and efficient isolation of nucleic acids from a variety of swab types and stool samples.
Purified viral RNA and DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Features and Benefits
- Fast and efficient purification: no lysis incubation and minimal hands-on, preparation steps
- Higher quality nucleic acids: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents
- Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Other Notes
Legal Information
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Artículos
Efecto del método de purificación en la precisión de la cuantificación del ADN y en los procesos enzimáticos posteriores Evaluación de la pureza del ADN genómico mediante espectrofotometría UV, electroforesis en gel y qPCR consecutiva utilizando los kits de purificación de ADN GenElute™-E.
GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.
Impact de la méthode de purification sur l'exactitude de la quantification de l'ADN et les procédés enzymatiques en aval. Évaluation de la pureté de l'ADN génomique par spectrophotométrie UV, électrophorèse sur gel et qPCR en aval à l'aide des kits de purification d'ADN GenElute™-E.
Contenido relacionado
Reduzca los residuos de plástico y elimine los residuos líquidos peligrosos para laboratorios más sostenibles con los kits de preparación de ADN y ARN con centrifugado único GenElute™-E.
Answers to frequently asked questions related to GenElute™-E single spin DNA and RNA purification and negative chromatography
Réduisez les déchets plastiques et évitez les déchets liquides dangereux pour des laboratoires plus éco-responsables grâce aux kits de préparation d'ADN et d'ARN GenElute™-E Single Spin.