The recommendation to store this product at -20°C is due to the presence of 50% glycerol, which lowers its freezing point to approximately -23°C. Consequently, it should not freeze at -20°C; if it does, it may indicate that the freezer is set too low, not properly calibrated, or that the glycerol ratio is incorrect. If the material is frozen at or below -23°C, or if it contains less or no glycerol, the gel could be damaged, become fragile, and fail to perform as expected.
E6406
EZview™ Red Glutathione Affinity Gel
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 354,00 € |
5 x 1 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 684,00 € |
Acerca de este artículo
354,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleanalyte chemical class(es)
proteins (GST)
Quality Segment
technique(s)
immunoprecipitation (IP): suitable, protein purification: suitable
storage temp.
2-8°C
General description
EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.
Application
The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
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| technique(s) immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| analyte chemical class(es) proteins (GST) | analyte chemical class(es) proteins (Immunoglobulins of various mammalian species) | analyte chemical class(es) proteins (Immunoglobulins of various mammalian species) | analyte chemical class(es) - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
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Why should E6406 be stored at -20°C, and what could happen if it is stored at a colder temperature?
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