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DNAQF

Sigma-Aldrich

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

Sinónimos:

dsDNA quantification

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About This Item

Código UNSPSC:
41105500
NACRES:
NA.84

Nivel de calidad

200

uso

sufficient for 750 assays (2 ml each)

técnicas

nucleic acid detection: suitable

temp. de almacenamiento

−20°C

Descripción general

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

Aplicación

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
  • in neonatal rat ventricular myocytes (NRVMs)
  • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
  • in mouse embryonic fibroblast cell line (NIH3T3 cells)

Características y beneficios

  • Specific for the quantitation of nanogram amounts of DNA
  • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Solo componentes del kit

Referencia del producto
Descripción
  • Detailed technical bulletin 1 ea

Producto relacionado

Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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