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Merck

D7679

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System

liquid

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Número CAS:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:

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InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

form

liquid

technique(s)

immunoblotting: suitable

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

Quality Level

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D7304D6815D5637
solubility

water: soluble

solubility

-

solubility

-

solubility

H2O: 50 mg/mL

technique(s)

immunoblotting: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: 96-104% (with NaOH)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

powder, crystals or chunks

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

General description

DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.

Preparation Note

Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.

Disclaimer

Stable at least one year at 2-8 °C.

Solo componentes del kit

Referencia del producto
Descripción

  • 10× DAB Liquid Chromogen 10 mL

  • premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9

wgk

WGK 3

Clase de almacenamiento

3 - Flammable liquids

pictograms

Flame

signalword

Danger

hcodes

Hazard Classifications

Flam. Liq. 2

flash_point_f

51.8 °F - closed cup

flash_point_c

11 °C - closed cup


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Carola W N Damen et al.
Analytical biochemistry, 393(1), 73-79 (2009-06-16)
For the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (ELISAs)--are the most widely used techniques. Although ELISAs are very sensitive, the obtained sensitivity is not always sufficient. In this study, we have investigated the possibilities
The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm
Smith AN, et al.
Developmental Biology, 285(2), 477-489 (2005)
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in gastrointestinal system
Jin SY, et al.
World Journal of Gastroenterology, 12(7), 1092-1092 (2006)
N M Chu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37(2), 257-263 (1989-02-01)
Immunoblotting techniques are widely used for detection of antigen immobilized on nitrocellulose membranes. There are many immunolabeling methods and staining methods available to disclose the presence of antigen in such techniques. Five common staining methods each for alkaline phosphatase and

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