Saltar al contenido
Merck
Todas las fotos(4)

Documentos clave

Ver Toda la documentación

D3514

Sigma-Aldrich

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate

≥80% (elemental analysis), powder

Sinónimos:

DIDS, Disodium 4,4′-diisothiocyanatostilbene-2,2′-disulfonate

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(4)

About This Item

Fórmula empírica (notación de Hill):
C16H8N2Na2O6S4 · xH2O
Número de CAS:
207233-90-7
Peso molecular:
498.48 (anhydrous basis)
Beilstein:
8179433
Número MDL:
MFCD00009638
Código UNSPSC:
12352200
ID de la sustancia en PubChem:
24893761
NACRES:
NA.25
Para consultas sobre este producto D3514, póngase en contacto con su oficina o distribuidor locales de Merck. Póngase en contacto con el Servicio técnico

grado

for analytical purposes

Ensayo

≥80% (elemental analysis)

Formulario

powder

idoneidad de la reacción

reagent type: cross-linking reagent

color

yellow

solubilidad

0.1 M potassium bicarbonate: 50 mg/mL

temp. de almacenamiento

2-8°C

cadena SMILES

[Na+].[Na+].[O-]S(=O)(=O)c1cc(ccc1\C=C\c2ccc(cc2S([O-])(=O)=O)N=C=S)N=C=S

InChI

1S/C16H10N2O6S4.2Na/c19-27(20,21)15-7-13(17-9-25)5-3-11(15)1-2-12-4-6-14(18-10-26)8-16(12)28(22,23)24;;/h1-8H,(H,19,20,21)(H,22,23,24);;/q;2*+1/p-2/b2-1+;;

Clave InChI

GEPAYBXVXXBSKP-SEPHDYHBSA-L

¿Está buscando productos similares? Visita Guía de comparación de productos

Aplicación

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate has been used:
  • as band3 protein inhibitor in rabbits(19)
  • for the inhibition of bicarbonate transporters in brain slice tissue(20)
  • as HCO3-/Cl- exchanger (anion exchanger) in embryos(21)

A negatively charged homobifunctional cross-linking reagent. The isothiocyanate groups react with primary amines at pH 9.0-10.0.

Acciones bioquímicas o fisiológicas

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid is an anionic alkylating agent containing isothiocyanate residue.[1] It is a negatively charged homobifunctional cross-linking reagent.[2] 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid is a specific inhibitor of cellular anion permeability used in cell transport studies.[3] The isothiocyanate groups react with primary amines at pH 9.0-10.0. 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid alkylates lysine residues and inhibits apoptosis indirectly by targeting membrane based anion transporters.[1]In addition, it also inhibits calcium transport in vesicles.[4]
A specific inhibitor of cellular anion permeability used in cell transport studies.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
MKCN4279
MKCG5622
MKCD5234
MKBX6796V
MKBT1609V

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Michael Kittl et al.
International journal of molecular sciences, 20(14) (2019-07-18)
Many cell types express an acid-sensitive outwardly rectifying (ASOR) anion current of an unknown function. We characterized such a current in BV-2 microglial cells and then studied its interrelation with the volume-sensitive outwardly rectifying (VSOR) Cl- current and the effect
Narongrit Thongon et al.
Pflugers Archiv : European journal of physiology, 468(11-12), 1809-1821 (2016-11-21)
Hypomagnesemia is the most concerned side effect of proton pump inhibitors (PPIs) in chronic users. However, the mechanism of PPIs-induced systemic Mg2+ deficit is currently unclear. The present study aimed to elucidate the direct effect of short-term and long-term PPIs
Andrew A Kelso et al.
Molecular and biochemical parasitology, 210(1-2), 71-84 (2016-09-30)
The protozoan parasite responsible for human amoebiasis is Entamoeba histolytica. An important facet of the life cycle of E. histolytica involves the conversion of the mature trophozoite to a cyst. This transition is thought to involve homologous recombination (HR), which
Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain
Fukui K, et al.
Nucleic Acids Research, 35(3), 850-860 (2007)
Egor Turovsky et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(42), 10750-10758 (2016-11-01)
Ventral regions of the medulla oblongata of the brainstem are populated by astrocytes sensitive to physiological changes in PCO2/[H+]. These astrocytes respond to decreases in pH with elevations in intracellular Ca2+ and facilitated exocytosis of ATP-containing vesicles. Released ATP propagates
Ver todos los artículos relacionados

Preguntas

1–8 de 8 Preguntas  

  1. Are effects on membrane transport due to addition of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt treatment reversible? i.e., can DIDS be 'washed out'?

    1 respuesta

    1. Unfortunately, this product has not been studied to determine reversibility. An older publication (see link #1) suggests that both reversible and irreversible binding occurs, while a more recent paper (see link #2) suggests that the process is irreversible. A further study of the literature would be recommended.

      Publication #1:
      https://link.springer.com/article/10.1007/BF01868957

      Publication #2:
      https://www.nature.com/articles/cddiscovery201537

      ¿Le ha resultado útil?

  2. What are the fluorescent properties of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    1 respuesta

    1. Product No. D3514 has an excitation maximum at a wavelength of 342 nm, and an emission maximum at a wavelength of 418 nm, measured in water.

      ¿Le ha resultado útil?

  3. How does the storage temperature relate to shipping conditions?

    1 respuesta

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      ¿Le ha resultado útil?

  4. What is the Department of Transportation shipping information for this product?

    1 respuesta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      ¿Le ha resultado útil?

  5. Is there a reference for the use of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514) as a cross-linking reagent?

    1 respuesta

    1. Product No. D3514 binds covalently (irreversibly) to the outer surface of human erythrocyte membrane protein, functioning as an anion transport inhibitor. It also can be used to cross-link membrane anion transport sites.  A good cross-linking reference is J. Membrane Biol., 29, 147-177 (1976).

      ¿Le ha resultado útil?

  6. What is the solution stability of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    1 respuesta

    1. We have no specific solution stability data for Product No. D3514, however, isothiocyanates are unstable in water and should not be stored in aqueous solutions.

      ¿Le ha resultado útil?

  7. Is there a reference for the use of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514) as a cross-linking reagent?

    1 respuesta

    1. The spectral properties of Product No. D3514 are: ?max: 220 nm, 265 nmExtinction coefficient: EmM = 3000 (280 nm, water), EmM = 54 (342 nm, water).  This information is from J. Membrane Biol., 29, 147-177 (1976).

      ¿Le ha resultado útil?

  8. What is the solubility of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    1 respuesta

    1. Product No. D3514 is soluble in 0.1 M KHCO3 (50 mg/mL) yielding a hazy, yellow-green solution. This may require heat for complete solubilization. DIDS is also soluble in DMSO.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico