The molecular weight for this product is not determined. However, other sources report a molecular weight of 24,000 daltons for Diaphorase from Clostridium.
D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Sinónimos:
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 76,90 € |
300 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 211,00 € |
500 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 268,00 € |
76,90 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlerecombinant
expressed in E. coli
Quality Segment
form
solid
specific activity
>=30 units/mg protein (biuret)
storage temp.
−20°C
General description
Research area: Cancer
Application
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.
- impregnated on the strip for formate dehydrogenase assay.[1]
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.[2]
- for evaluation of glucose uptake in cultured muscle cells.[3]
- in lactate dehydrogenase cytotoxicity assay.
Biochem/physiol Actions
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Physical form
Supplied as a lyophilized powder containing trehalose.
Other Notes
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
1 of 1
Este artículo | |||
|---|---|---|---|
| specific activity >=30 units/mg protein (biuret) | specific activity 3.0-20.0 units/mg protein (biuret) | specific activity ~20 units/mg protein (At 25 °C with acetaldehyde as the substrate.) | specific activity ≥600 units/mg protein (biuret) |
| form solid | form lyophilized powder | form lyophilized | form ammonium sulfate suspension |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
What is the molecular weight of this diaphorase?
1 respuesta-
¿Le ha resultado útil?
-
-
How to prepare stock solution for Diaphorase in order to use 2 units in reaction
1 respuesta-
For assay purposes, this product is reconstituted 1 mg/ml in cold 200 mM Tris-HCl buffer containing 294 mM Potassium Chloride, 0.54 mM Riboflavin 5'-monophosphate, and 0.025% (w/v) BSA, pH 7.5. The minimum activity of this enzyme is 30 u/mg protein with a minimum protein content of 50%. At the minimum activity and minimum protein content, 2 units of 1 mg/mL stock solution would have a volume of 133 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d2197#product-documentation¿Le ha resultado útil?
-