LB205

98062329, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• product name: LB205, 98062329
• origen biológico: human blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed

Descripción línea celular

Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.

Aplicación

DNA repair deficiency studies, hprt mutation analysis

Medio de cultivo

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Otras notas

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