JVM-13

96090516, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• product name: JVM-13, 96090516
• origen biológico: human blood
• modo de crecimiento: Suspension
• cariotipo: 46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian B-prolymphocytic leukaemia, EBV transformed

Descripción línea celular

Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.

Aplicación

Study of B cell leukaemia

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18

Medio de cultivo

RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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