AT-2.1

94101450, rat prostate (ventral), Epithelial-like

• Código UNSPSC: 41106514

Propiedades

• product name: AT-2.1, 94101450
• origen biológico: rat prostate (ventral)
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat prostatic cancer

Descripción línea celular

AT-2.1 was developed via in vivo passages of the Dunning R-3327 rat prostatic cancers. A series of other cell lines were produced and they hold in common with Dunning R-3327 and AT-2.1 a structural change in chromosome 4 involving a translocation.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/ml. Cells can be easily dislodged from the monolayer mechanically, however 0.25% trypsin or trypsin/EDTA can be used; 5% CO₂; 37°C.

Otras notas

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