IPI-1

93100621, Porcine intestine, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• product name: IPI-1, 93100621
• origen biológico: Porcine intestine
• modo de crecimiento: Adherent
• cariotipo: Heteroploid
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Boar Miniature male ileum, SV40 transformed

Descripción línea celular

Fibroblast cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo.

Aplicación

Potential feeder cell line for the recovery of epithelial cells isolated from the small intestine.

Medio de cultivo

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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