C0146

93021839, from human blood

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human blood
• Número panel HRC: 3
• etnicidad: UK Caucasian
• género: female
• modo de crecimiento: Suspension
• cariotipo: Not Specified
• morfología: Lymphoblastoid
• técnicas: cell culture | mammalian: suitable

Descripción

Origen línea celular

UK Caucasian Female

Descripción línea celular

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

Aplicación

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Medio de cultivo

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutina de subcultivo

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

Otras notas

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