1BR.3.N

90020508, human skin, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: 1BR.3.N, 90020508
• origen biológico: human skin
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human skin fibroblast, transformed

Descripción línea celular

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV2neo. The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

Aplicación

DNA repair and cell growth studies

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 9,12
D16S539: 12,14
D5S818: 12
D7S820: 8,10
THO1: 9.3
TPOX: 11
vWA: 14,15

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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