3T3(+3)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse unknown/unspecified
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast

Propiedades

• biological source: mouse unknown/unspecified
• packaging: tube of 5 μg 89022402-DNA-5UG, pkg of vial of cells 89022402-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Biochem/physiol Actions

Please note: Available stocks of this cell line are not contact-inhibited. Contact inhibition is the natural process of arresting cell growth when cells come into contact with each other. It is not possible to be sure how the loss of contact inhibition may affect other characteristics of the cell line. This cell line catalog number continues to be a popular choice.

The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.

Mouse

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].