KHOS-240S

86112810, human bone, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: KHOS-240S, 86112810
• origen biológico: human bone
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, aneuploid
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian osteosarcoma

Descripción línea celular

A revertant line of the KHOS/NP cell line (Sigma Catalogue number. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.

Aplicación

Tumourigenicity, transformation, biochemical and virus studies: MSV

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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