RAJI Cell Line human

85011429, human lymph, Lymphoblastoid

• Sinónimos: GM04671 Cells, P1-Raji Cells
• Código UNSPSC: 41106514

Propiedades

• product name: RAJI Cell Line human, 85011429
• origen biológico: human lymph
• descripción: Human Black, Burkitt′s lymphoma
• modo de crecimiento: Suspension
• cariotipo: 2n = 46, diploid
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Burkitt′s lymphoma

Descripción línea celular

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Aplicación

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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