HF19

85011418, human lung, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• product name: HF19, 85011418
• origen biológico: human lung
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human foetal lung fibroblast

Descripción línea celular

HF19 has been established from a 14 week old female foetus. The cells are heterozygous for thymidine kinase and have been used for studies of radiation-induced DNA damage.

Aplicación

DNA repair studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells /cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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